DYNAMIC CONTROL OF HYDROGEL PROPERTIES VIA ENZYMATIC REACTIONS Dustin Michael Moore 10.25394/PGS.8025614.v1 https://hammer.purdue.edu/articles/thesis/DYNAMIC_CONTROL_OF_HYDROGEL_PROPERTIES_VIA_ENZYMATIC_REACTIONS/8025614 Two Systems were designed. The first permits tunable on-demand softening of a hydrogel network. The second permits reversible on demand ligand exchange within a hydrogel network. Both means were shown to be cytocompatible and their uses demonstrated in cell culture of mesenchymal stem cells and 3T3 fibroblast cells. 2019-06-10 20:37:25 Tissue engineering hydrogel softening Sortase A Ligand Exchange Mesenchymal Stem Cells 3T3 Fibroblast Cells Biomechanical Engineering