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FAT AND SODIUM QUANTIFICATION AND CORRELATION BY MRSI

thesis
posted on 16.06.2020 by Ahmad Abdurahman M. Alhulail

Lipids and sodium (23Na) are two essential components of the human body. They play a role in almost all biological systems. However, an increase in their levels is associated with metabolic diseases. The elevation of their contents can cause similar health disorders. Examples of prevalent disorders that share an increase of musculoskeletal lipids and 23Na are hypertension and diabetes. However, the relationship between in vivo lipid and sodium levels in pathophysiology has not been studied enough and therefore is still unclear. Additionally, the available quantification methods to facilitate such a study may not be practical. They are either invasive, not sensitive enough, or require an impractical measurement time.

Therefore, in this work, our aims were to develop practical in vivo methods to quantify the absolute sodium concentration as well as the concentration of each lipid component individually, and to study the correlation between them within the skeletal muscles.

Since lipids and 23Na have different nuclear magnetic resonance properties, their quantification by magnetic resonance (MR) techniques face different challenges. Thus, we optimized different MR spectroscopic imaging (MRSI) techniques for lipids and 23Na.

Our proposed proton MRSI was able to provide eight lipid fat fraction (FF) maps representing each musculoskeletal lipid component (fatty acid) detected by our MRSI technique, and demonstrated a superior sensitivity compared to the conventional MR imaging methods.

For 23Na, our developed 23Na-MRSI was able to measure and map the absolute 23Na concentration with values agreeing with those reported previously in biopsy studies, and with a high repeatability (CV < 6 %) within significantly shorter acquisition time compared to other available techniques.

Finally, the 23Na concentration and the fat fractions of each lipid component within healthy skeletal muscles were measured and correlated using our developed MRSI methods. Our findings suggest a positive regional relationship between 23Na and lipids and negative correlation between 23Na and BMI under healthy conditions.

Funding

Indiana Clinical and Translational Sciences Institute

National Institutes of Health (UL1TR001108)

National Center for Advancing Translational Sciences; Clinical and Translational Sciences Award

College of Health and Human Sciences, Purdue University (pilot grant)

History

Degree Type

Doctor of Philosophy

Department

Health Science

Campus location

West Lafayette

Advisor/Supervisor/Committee Chair

Dr. Ulrike Dydak

Additional Committee Member 2

Dr. Uzay Emir

Additional Committee Member 3

Dr. Joseph Rispoli

Additional Committee Member 4

Dr. Keith Stantz

Licence

Exports